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15N-labed glycine synthesis Anais da ABC (AABC)
Tavares,Claudinéia R.O.; Bendassolli,José A.; Coelho,Fernando; Sant'ana Filho,Carlos R.; Prestes,Clelber V..
This work describes a method for 15N-isotope-labeled glycine synthesis, as well as details about a recovery line for nitrogen residues. To that effect, amination of alpha-haloacids was performed, using carboxylic chloroacetic acid and labeled aqueous ammonia (15NH3). Special care was taken to avoid possible 15NH3 losses, since its production cost is high. In that respect, although the purchase cost of the 13N-labeled compound (radioactive) is lower, the stable tracer produced constitutes an important tool for N cycling studies in living organisms, also minimizing labor and environmental hazards, as well as time limitation problems in field studies. The tests were carried out with three replications, and variable 15NH3(aq) volumes in the reaction were used...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stable isotope; Glycine; Amino acid.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000300005
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A leucine-supplemented diet improved protein content of skeletal muscle in young tumor-bearing rats BJMBR
Gomes-Marcondes,M.C.C.; Ventrucci,G.; Toledo,M.T.; Cury,L.; Cooper,J.C..
Cancer cachexia induces host protein wastage but the mechanisms are poorly understood. Branched-chain amino acids play a regulatory role in the modulation of both protein synthesis and degradation in host tissues. Leucine, an important amino acid in skeletal muscle, is higher oxidized in tumor-bearing animals. A leucine-supplemented diet was used to analyze the effects of Walker 256 tumor growth on body composition in young weanling Wistar rats divided into two main dietary groups: normal diet (N, 18% protein) and leucine-rich diet (L, 15% protein plus 3% leucine), which were further subdivided into control (N or L) or tumor-bearing (W or LW) subgroups. After 12 days, the animals were sacrificed and their carcass analyzed. The tumor-bearing groups showed a...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Leucine; Amino acid; Walker 256 carcinosarcoma; Body composition; Skeletal muscle protein.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100017
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Arginine improves nutritional quality of sow milk and piglet performance R. Bras. Zootec.
Moreira,Rennan Herculano Rufino; Lanferdini,Eloiza; Fonseca,Leonardo da Silva; Chaves,Rhuan Filipe; Garbossa,Cesar Augusto Pospissil; Saraiva,Alysson; Nogueira,Eduardo Terra; Abreu,Márvio Lobão Teixeira de.
ABSTRACT The objective of this study was to evaluate the effect of L-arginine supplementation in lactation diets on the productive and reproductive performance of pluriparous sows and their litters. Seventy-six sows of the same genetic line were assigned to four treatments in a completely randomized design with 19 replicates. The experimental unit was the sow and its respective litter. Treatments comprised a control diet (no L-arginine supplementation) and other three diets obtained by top dressing the control diet with 0.5, 1.0, and 1.5% of L-arginine. L-arginine supplementation had no effect on any performance variables, body condition, milk production, or weaning-estrus interval. There was a quadratic effect on percentage of protein and fat in milk as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Lactation; Milk composition; Reproduction; Swine.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100530
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BONE DEVELOPMENT OF BROILER CHICKENS FED DIETS WITH DIFFERENT AMINO ACID AND CALCIUM LEVELS DURING THE STARTER PHASE International Journal of Morphology
Soares da Silva Araújo,Cristiane; Martinez Baraldi Artoni,Silvana; Francelino Araújo,Lúcio; Jeferson Quirino Lousada,Mário; Mack Junqueira,Otto; Roque Rodriguez,Anita Isabel.
Two experiments were conducted in order to evaluate the effects of different dietary amino acid and calcium profiles on the bone characteristics of two chicken strains (Avian Farms and Cobb) during the starter phase. At the end of each experiment, 2 chickens of each replicate were sacrificed and their tibiae were collected for analysis of following bone characteristics: bone measurements (weight, length and thickness of the compact and spongy layers) and bone density (bone densitometry). A total of 540 birds were used in each experiment, divided into fully randomized blocks with a 3 x 2 factorial schemes, i. e., 3 amino acid profiles (methionine, lysine and threonine ­ 100%, 125% and 150% of NRC recommendations, 1994) and 2 calcium levels (75% and...
Tipo: Journal article Palavras-chave: Amino acid; Bone density; Broiler chickens; Calcium.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000200001
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Chemical composition and fatty acid contents in farmed freshwater prawns PAB
Portella,Carolina de Gasperi; Sant'Ana,Léa Silvia; Valenti,Wagner Cotroni.
The objective of this work was to evaluate the chemical composition and fatty acid contents of Amazonian and giant river prawns. After four-month farming, with the same diet for both species, palmitic and stearic acids were the main saturated fatty acids. Oleic acid was the main monounsatured fatty acid, and the eicosapentaenoic and docosahexaenoic acids were the most abundant polyunsaturated acids. Amazonian prawn has higher levels of protein and polyunsaturated fatty acids than those of the giant river prawn, which shows its potential for aquaculture.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Macrobrachium amazonicum; Macrobrachium rosenbergii; Amino acid; Fatty acids; Meat quality.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800046
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Digestibility of animal and vegetable protein ingredients by pirarucu juveniles, Arapaima gigas R. Bras. Zootec.
Cipriano,Filipe dos Santos; Lima,Kauana Santos de; Souza,Ricardo Henrique Bastos de; Tonini,William Cristiane Teles; Passinato,Érica Bevitório; Braga,Luís Gustavo Tavares.
ABSTRACT The objective of this study was to determine the apparent digestibility coefficients of energy, protein, and amino acids in protein ingredients by pirarucu juveniles. A test was conducted with six protein ingredients: meat and bone meal, fish meal, hydrolyzed feather meal, poultry by-product meal, soybean meal, and corn gluten meal. Three repetitions were used for each tested ingredient. A reference feed was used with 430 g kg−1 crude protein and 19.63 kJ g−1 gross energy. The test feeds consisted of the replacement of 30% of the reference feeds with the test ingredients. Chromium oxide was added to the feeds at 1 g kg−1 as an external marker. Eighteen juveniles with an average weight of 235±36 g were used. The best apparent digestibility...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Carnivore species; Fish nutrition; Nutritional value.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016001000581
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Digestible lysine and organic zinc for male broiler from 1 to 11 days of age: performance and body composition R. Bras. Zootec.
Trindade Neto,Messias Alves da; Namazu,Lilian Bernadete; Kobashigawa,Estela; Takeara,Paula; Donato,Daniella Carolina Zanardo; Albuquerque,Ricardo de.
This study evaluated levels of digestible lysine and organic zinc for male Ross strain broilers from 1 to 11 days of age. It was used 1,050 chicks distributed in randomized block design, in 5 × 2 factorial scheme, with seven repetitions of 15 birds per experimental unit. The dietary concentrations of digestible lysine were 0.90; 1.00; 1.10; 1.20; and 1.40% combined with 43 and 253 ppm zinc chelate. The diets contained 2,965 ± 18 kcal/kg of apparent metabolizable energy (AME) and 21.48 ± 0.18% of CP. It was determined chemical composition, protein, lipid, mineral and water depositions on carcass and empty body. There was no interaction among the factors digestible lysine and organic zinc. Effect of zinc concentration increase was observed on greatest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Nutrient deposition; Nutrition; Requirement; Zinc.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000300019
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Digestible lysine levels in diets supplemented with ractopamine R. Bras. Zootec.
Souza,Evelar de Oliveira; Haese,Douglas; Kill,João Luís; Haddade,Ismail Ramalho; Lacerda,Elcio das Graça; Saraiva,Alysson; Silva,Francisco Carlos de Oliveira; Sobreiro,Rodrigo Pereira.
In order evaluate digestible lysine levels in diets supplemented with 20 ppm of ractopamine on the performance and carcass traits, 64 barrows with high genetic potential at finishing phase were allotted in a completely randomized block design with four digestible lysine levels (0.80, 0.90, 1.00, and 1.10%), eight replicates and two pigs per experimental unit. Initial body weight and pigs' kinship were used as criteria in the blocks formation. Diets were mainly composed of corn and soybean meal supplemented with minerals, vitamins and amino acids to meet pigs' nutritional requirements at the finishing phase, except for digestible lysine. No effect of digestible lysine levels was observed in animal performance. The digestible lysine intake increased linearly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Additive; Amino acid; Carcass; Diet; Meat.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011001000018
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Digestible Lysine on Live Performance of Chicken Type Naked Neck During the Starter Phase Rev. Bras. Ciênc. Avic.
Oliveira,RG de; Pinheiro,SRF; Castro,MR de; Vieira,DJ; Pires,AV; Abreu,LRA de.
ABSTRACT The poultry market has changed due to a higher consumer interest on products with differentiated organoleptic characteristics, making of free-range broiler production a promising activity. This experiment was conducted to determine the digestible lysine requirements of Redbro Cou Nu male and female chickens during the starter phase (one to 21 days of age). Six hundred and thirty Redbro Cou Nu broilers were distributed into 30 pens (21 chickens/pen) according to a randomized complete design in a 5 x 2 factorial arrangement, consisting of five levels of digestible lysine and two sexes, with three replicates (pens) per treatments. Diets with increasing digestible lysine levels (8.1, 9.5, 10.9, 12.3 and 13.7 g of digestible lysine per kg of diet) were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Feed conversion ratio; Ideal protein; Nutritional requirement.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000400483
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Digestible Lysine Requirements the Performance, Carcass Traits and Breast Meat Quality of Slow-Growing Broilers Rev. Bras. Ciênc. Avic.
Brasil,RJM; Lima,CAR; Machado,NJB; Curvello,FA; Quaresma,DV; Vieites,FM; Sousa,FDR.
ABSTRACT Three experiments were conducted to estimate the digestible lysine requirements of slow-growing broilers on their performance, carcass traits, and breast meat quality. Different broilers were evaluated in each experiment. In Experiment 1, broilers were evaluated from 29-49 days of age (grower phase I), in Experiment 2, from 50 to 69 days old (grower phase II), and in Experiment 3, from 70-84 days old (finisher phase). A completely randomized design with five treatments of four replicates each was applied in all experiments. The following dietary digestible lysine values were investigated: 0.871, 1.011, 1.151, 1.291 and 1.431% in Experiment 1; 0.803, 0.943, 1.083, 1.223 and 1.363% in Experiment 2; and 0.766, 0.906, 1.046, 1.186 and 1.326% in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Meat quality; Lysine requirements; Slow-growing broilers.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300555
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Digestible threonine for slow-growing broilers: performance, carcass characteristics, intestinal mucin, and duodenal morphometry R. Bras. Zootec.
Machado,Noédson de Jesus Beltrão; Lima,Cristina Amorim Ribeiro de; Brasil,Ronner Joaquim Mendonça; Quaresma,Débora Vaccari; Dilelis,Felipe; Silva,Ana Paula Pereira da; Curvello,Fernando Augusto.
ABSTRACT Five experiments were conducted to estimate the digestible threonine requirements of slow-growing broilers: experiment 1, starter phase (days 10 to 28); experiment 2, grower I phase (days 29 to 49); experiment 3, grower II phase (days 50 to 69); experiment 4, finisher phase (days 70 to 84); and experiment 5, which was specifically conducted to determine the production of intestinal mucin over two periods (days 50 to 69 and 70 to 84). Different birds were used in all experiments. A completely randomized design with five treatments and four replicates was used. Treatments consisted of increasing the values of digestible threonine in the diet through basal feed supplementation with L-threonine (98.5%), which was added instead of cornstarch. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Nutritional requirements; Villus.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100514
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Digestible tryptophan:digestible lysine ratio in diets for laying hens from 24 to 40 weeks of age R. Bras. Zootec.
Calderano,Arele Arlindo; Gomes,Paulo Cezar; Donzele,Juarez Lopes; Barreto,Sergio Luiz de Toledo; Vellasco,Cássia Rampini; Mello,Heloisa Helena de Carvalho; Lelis,Guilherme Rodrigues; Rocha,Tatiana Cristina da.
The objective of this study was to determine the ideal digestible tryptophan:digestible lysine ratio in diets for laying hens from 24 to 40 weeks of age. Two hundred and forty Hy-Line W-36 laying hens at 24 weeks of age were distributed in a completely randomized design, with five treatments, eight replicates and six birds per experimental unit. The digestible tryptophan levels in the experimental diets were 1.57; 1.68; 1.79; 1.90 and 2.01 g/kg, providing ratios of digestible tryptophan:digestible lysine of 0.215; 0.230; 0.245; 0.260 and 0.275. The increase in the levels of digestible tryptophan in the diet linearly improved the feed intake, digestible tryptophan intake, digestible lysine intake, egg production, egg mass, feed conversion per egg mass and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Birds; Ideal protein.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000006
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DIGESTIBLE VALINE REQUIREMENTS IN LOW-PROTEIN DIETS FOR BROILERS CHICKS Rev. Bras. Ciênc. Avic.
Nascimento,GR; Murakami,AE; Ospina-Rojas,IC; Diaz-Vargas,M; Picoli,KP; Garcia,RG.
ABSTRACT Three experiments were carried out to evaluate the levels of digestible valine in diets with reduced crude protein on the performance, carcass yield and muscle fiber diameter of male broilers during the pre-starter (1 to 8 d of age), starter (9 to 21 d of age) and grower phases (21 to 42 d of age). A total of 1,080 chickens in the pre-starter phase, 900 in the starter phase and 864 in the grower phase were distributed in a completely randomized design with six treatments, consisting of a control positive diet (C+) and diets with 2 percentage points (p.p.) reduction of crude protein level and five graded levels of digestible valine (Val), with six replicates of 30, 25 and 24 birds per experimental unit, respectively. The recommended level of TID...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Branched-chain; Fiber diameter.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000300381
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Effect of dietary amino acid composition from proteins alternative to fishmeal on the growth of juveniles of the common snook, Centropomus undecimalis R. Bras. Zootec.
Silvão,Cristiane Freire; Nunes,Alberto Jorge Pinto.
ABSTRACT This study investigated the effect of dietary amino acid composition from proteins alternative to fishmeal on the growth performance of the common snook, Centropomus undecimalis. Fish of 10.79±0.71 g (n = 150) were stocked in 15 shaded outdoor tanks of 1 m3. The basal diet contained 643.4 g kg−1 salmon byproduct meal (SML) and 200.0 g kg−1 soy protein concentrate (SPC). Two other diets replaced 39 and 29% of the SML with poultry byproduct meal (PBM, 170.1 g kg−1) and SPC (334.9 g kg−1), respectively. Fish were fed twice daily for 84 days under 32±1 g L−1 water salinity and 27.3±0.9 °C temperature. Final survival (99.5±2.6%) was unaffected by dietary treatment. Snook grew slower (0.24±0.03 and 0.27±0.04 vs 0.35±0.06 g day−1) and achieved the lowest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Fishmeal; Fish nutrition; Nutritional requirement.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000700569
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Effect of energy levels of diets formulated on total or digestible amino acid basis on broiler performance Rev. Bras. Ciênc. Avic.
Maiorka,A; Dahlke,F; Santin,E; Kessler,AM; Penz Jr.,AM.
This study was conducted to evaluate the performance and carcass and breast yields of broilers fed two different energy levels (3,200 or 2,900 kcal ME/kg) and two ways of expressing amino acid requirements (total - TAA or digestible - DAA) between 21 and 42 days of age. The results showed that broilers fed the diet containing 3,200 kcal ME/kg had higher weight gain (P=0.015), better feed conversion (P=0.001), and higher abdominal fat deposition (P=0.001) as compared to those fed the diet containing 2,900 kcal ME/kg. Diets formulated on DAA basis promoted higher weight gain (P=0.043), better feed conversion (P=0.010) and better conversion of ME intake into weight gain (P=0.007) as compared to those formulated on TAA basis. The results of this study suggest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Broilers; Energy.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200003
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Effects of crossbreeding on slaughter traits and breast muscle chemical composition in chinese chickens Rev. Bras. Ciênc. Avic.
Huang,Ai-xia; Li,Jin-jun; Shen,Jun-da; Tao,Zheng-rong; Ren,Jin-dong; Li,Guo-qing; Wang,De-qian; Tian,Yong; Yang,Fu-sheng; Ding,Wei-ping; Wu,Tian-xing; Lu,Li-zhi.
We investigated the effects of crossbreeding on slaughter traits and the chemical composition of chicken breast muscle. Trials were conducted using 120 broilers from four lines: Xiao-Shan chicken (XS), Xian-Ju chicken (XJ), Xiao-Shan chicken♂♂ × Xian-Ju chicken♀♀ (Zhenan 1, ZNY1) and Xiao-Shan chicken♂♂ × (Guang-Xi Yellow chicken♂♂×Xian-Ju chicken♀♀) ♀♀ (Zhenan 2, ZNY2). The birds were slaughtered at 120 days of age and the slaughter traits were measured. Breast muscles were sampled to determine chemical composition. The slaughter traits of hybrid chickens were improved. Both hybrid strains had higher intramuscular fat (IMF) and inosine-5'-monophosphate (inosinic acid, IMP). Concentrations of monounsaturated fatty acids (MUFA) in breast muscles from the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Chicken; Crossbreeding; Fatty acid; Inosine-5'-monophosphate; Intramuscular fat; Slaughter traits.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2011000400005
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Effects of dietary lysine on broiler performance and carcass yield - meta-analysis Rev. Bras. Ciênc. Avic.
Mendes,AS; Api,I; Silva,L; Silva,RTL; Sausen,L; Menezes,LFG; Morello,GM; Carvalho,EH.
There has been a great evolution and improvement in the nutrition of poultry and lysine has been used the reference amino acid to increase animal protein deposition. Therefore, a meta-analysis was performed with the objective of studying the effect of different dietary lysine levels on the performance and carcass yield of broiler chickens. In total, 21 studies published in Brazil since 1999 were included. Bird performance considered daily weight gain (178 observations) and feed conversion ratio (188 observations) results. Carcass quality was evaluated relative to carcass yield (24 observations), abdominal fat (24 observations) and breast meat yield (30 observations). Data were checked for normality and were submitted to analysis of variance. Weight gain...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Nutrition; Poultry production.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000400013
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Effects of dietary valine:lysine ratios on lactation performance of primiparous sows nursing large litters R. Bras. Zootec.
Devi,Subramaniam Mohana; Park,Jae Won; Kim,In Ho.
ABSTRACT Eighteen sows were used to determine the effect of dietary valine:lysine (val:lys) ratios on lactation performance in primiparous sows and litter performance. Sows, raised in individual pens, were randomly assigned to 1 of 2 experimental diets containing different standardized ileal digestibility (SID) val:lys ratios of 0.80 or 0.85. Corn-soybean-wheat-based diets were formulated to achieve the dietary treatments (0.86% or 0.87% SID lysine, and 0.69% or 0.74% SID valine). All diets were formulated to have 3,450 kcal metabolizable energy/kg. The experiment lasted 24 d. At the end of the experiment, body weight loss, backfat loss, average daily feed intake, and days to return to estrus were not affected by the different dietary val:lys ratios....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Body weight; Feed intake; Pigs.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015001200420
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Effects of in ovo injection of lysine and methionine into fertile broiler (parent stock) eggs on hatchability, growth performance, caecum microbiota, and ileum histomorphology R. Bras. Zootec.
Coskun,Isa; Akkan,Aysenur; Erener,Guray.
ABSTRACT We investigated the effect of in ovo injection of lysine, methionine, or their mixture into fertile broiler eggs on hatchability, chick weight, growth performance, inner organ development, caecum total aerobic bacteria, E. coli, coliforms, Enterobactericaea, and ileal histomorphology of broilers. Three hundred fertile eggs obtained from 60-week-old Ross 308 broiler breeders were used. Before this study, a preliminary study was conducted to determine the optimal amino acid dose. Optimum amino acid dose was determined as 2 mg/0.2 mL. Before replacing hatching machine, eggs were weighed individually and numbered. On day 16 of incubation, these eggs were allocated to treatment groups: negative control (no injection), positive control (distilled water...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid; Broiler chick; In ovo feeding; Poultry; Ross 308.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100516
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Effects of various nutrients and renewal of medium on in vitro development of bovine oocytes matured and fertilized in vitro OAK
FUKUI, Yutaka; LEE, Eun Song; KANEZAWA, Yuka; 福井, 豊; 李, 殷松; 金澤, 由佳.
http://www.obihiro.ac.jp/~library/kenkyu.html
Palavras-chave: Amino acid; Glucose; In vitro development; Oocyte; Cattle.
Ano: 1995 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3790
Registros recuperados: 37
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